This data includes (1) Scripts to aggregate landscape resistance layers into squared and hexagonal grids (i.e., different representations and resolutions), (2) Input resistance layers and focal nodes in .txt format to run in Circuitscape (Python implementation v4.0.5). Circuitscape is a software tool for modeling and analyzing landscape connectivity, which simulates movement of organisms across landscapes by estimating resistance to movement across each point of the landscape. (3) Scripts for the ecological network analysis, and (4) environmental predictors for amphibian whole-life cycle habitats used to describe the local environment for BGI design (i.e. topographic, hydrologic, edaphic, vegetation, land-use derived, movement-ecology related).