# Nuclear microsatellite markers and genotype data for _Trichopria ddrosophilae_ This data set comprises (i) the characteristics of a set of 21 species-specific nuclear microsatellites for PCR amplification in _Trichopria drosophilae_ (ii) and genotype data for samples collected in southern Switzerland (Canton of Ticino), with few reference samples from Canton of Vaud, southern Germany, and northern Italy (lab-reared population). Markers were developed by Ecogenics GmbH, Balgach (Switzerland), using MiSeq Nano 2x250 v2 format (on a mix of 10 individuals). Multiplex PCR assays for multilocus genotyping were established by Ecological Genetics (WSL Birmensdorf), and population genetic analyses are found in Gugerli et al., Agrarforschung Schweiz 2019.