These data were used to generate graphs and tables for the Journal of Visualized Experiments (JoVE) publication 'Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method. In general, the data are generated as example data for optimization of this method. This dataset is associated with the following publications: Nelson, G., J. Guynn, and B. Chorley. Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method. Journal of Visualized Experiments. JoVE, Somerville, MA, USA, 127: e55911, (2017). Nelson, G., J. Currier, and B. Chorley. Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method. JoVE, Somerville, MA, USA, 2017.