The dataset contains the first draft genome sequence of the type strain of Mycobacterium chimaera, Fl-0169. This dataset is associated with the following publication: Pfaller, S., V. Tokarev, C. Kessler, C. McLimans, V. Gomez-Alvarez, J. Wright, D. King, and R. Lamendella. Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169. Genome Announcements. American Society for Microbiology, Washington, DC, USA, 5(8): e01620-16, (2017).