The Water Quality Dataset contains field and laboratory data that were collected at a field-scale permeable reactive barrier. Environmental samples were collected from groundwater monitoring wells and surface water. This dataset is associated with the following publication: Lawrinenko, M., L. Rhea, K. Forshay, T. Lee, M. White, and R. Wilkin. Long-term field study of nitrate and ammonium remediation using a permeable reactive barrier at a livestock feeding operation. JOURNAL OF ENVIRONMENTAL MANAGEMENT. Elsevier Science Ltd, New York, NY, USA, 380: 124962, (2025).